rt pcr buffer (TaKaRa)

TaKaRa is a verified supplier

TaKaRa manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

TaKaRa rt pcr buffer
Rt Pcr Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 25271 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcr+buffer/Premix+Ex+Taq/10__3390_slash_jof12040282-85-12-22
Average 99 stars, based on 25271 article reviews

rt pcr buffer - by Bioz Stars, 2026-10

99/100 stars

Images

Polymerase Chain Reaction:

Article Title: A randomized, double-blind, placebo-controlled trial of soluble corn fiber supplementation for children with asthma
Article Snippet: .. Each polymerase chain reaction (PCR) contained 2.5 μL of PCR buffer (TaKaRa, 10x concentration, 1x final), 1 μL of the Golay barcode tagged forward primer (10 μM concentration, 0.4 μM final), 1 μL of bovine serum albumin (ThermoFisher, 20 mg/mL concentration, 0.56 mg/μL final), 2 μL of dNTP mix (TaKaRa, 2.5 mM concentration, 200 μM final), 0.125 μL of HotStart ExTaq (TaKaRa, 5 U/μL, 0.625 U/μL final), 1 μL reverse primer (10 μM concentration, 0.4 μM final). ..

Article Title: Genome-wide development of genomic SSR markers, polymorphism analysis, and dosage-aware SSR fingerprinting in hexaploid Camellia Oleifera.
Article Snippet: .. Each locus was first amplified on an AB 2720 Thermal Cycler (Life Technologies Corporation, USA) in a 10 μL reaction containing 1× PCR buffer (Takara, Dalian, China), 2.0 mM MgCl2, 0.2 mM each dNTP, 0.2 μM forward primer, 0.2 μM reverse primer, 1 U Hot-Start Taq DNA polymerase (Takara, Dalian, China), and 1 μL template DNA (10 ng μL−1). ..

Article Title: Taxonomic review and molecular phylogenetics of the reef gobiid genus Fusigobius from Taiwan with comments on a new species from South China Sea.
Article Snippet: PCR was done in a Model 9700 thermal cycler (Perkin-Elmer), and 30–40 cycles were carried out. .. The 25 μL reaction volume contained 14.4 μL of sterile distilled water, 2.5 μL of 10× PCR buffer (Takara), 2.0 μL of dNTP (2.5 mM each), 2.5 μL of each primer (5 μM), 0.1 μL of 0.5 unit Ex Taq (Takara), and 1.0 μL of template. ..

Article Title: Exploring technical improvements for environmental nucleic acids-based biodiversity assessment and management in coastal ecosystems.
Article Snippet: Assessing and conserving marine biodiversity remain critical global challenges, particularly in highly disturbed coastal regions.. The use of environmental DNA (eDNA)-metabarcoding has revolutionized biodiversity assessment and management; however, the prevalence of both false positives and negatives continues to be a significant concern.. To address these technical errors, we tested two potential methodological improvements in the highly disturbed Guangdong-Hong Kong-Macao Greater Bay Area: (1) the use of random whole-genome amplification (WGA) to reduce false negatives derived from low eDNA concentration, and (2) the application of environmental RNA (eRNA)-metabarcoding to mitigate false positives arising from eDNA contamination by human activities.

Article Title: Breaking epigenetic shackles: targeting ARID1A methylation and the PI3K/AKT/mTOR-PD-L1 axis to overcome immune escape in gastric cancer
Article Snippet: .. The 50 uL MSP-PCR reaction mixture contained 5 μL of 10 × PCR Buffer (Mg 2 + Plus, 25 mM; R007A, TaKaRa Bio Inc., Shiga, Japan), 4 μL of dNTP Mixture (2.5 mM, R007A, TaKaRa, Shiga, Japan), 1 μL each of forward and reverse ARID1A primers (100 μM, Sangon Biotech, Shanghai, China), 0.25 μL of TaKaRa LA Taq HS DNA polymerase (5U/uL, R007A, TaKaRa, Shiga, Japan), 36.75 μL of nuclease-free H 2 O, and 2 μL of bisulfite-converted DNA template (50 ng/μL). .. Thermal cycling conditions for MSP amplification were as follows: Initial denaturation at 95 °C for 5 min, followed by 40 cycles of denaturation at 95 °C for 30 s, primer-specific annealing (59 °C for methylated [M] primers or 55 °C for unmethylated [U] primers) for 45 s, and extension at 72 °C for 45 s, with a final extension at 72 °C for 10 min. Methylation-specific PCR products were resolved on a 2% agarose gel pre-stained with YeaRed Nucleic Acid Gel Stain (YEASEN Biotechnology, Cat# 10202ES76, Shanghai, China) at 140 V for 40 min. DNA bands were visualized under UV light, and the 106 bp target fragments were identified by comparison with DL2000 Plus DNA Marker (MD101-01-AA; 100–5000 bp; Vazyme, Beijing, China).

Article Title: Mitochondrial Genome-Based Phylogeny of Turbellarians and Evidence for Accelerated Mitochondrial Evolution in Symbiotic Species
Article Snippet: .. The PCR reaction was performed in a 20 μl mixture containing 7.4 μl of double-distilled water, 10 μl of 2× PCR buffer (including Mg2+ and dNTPs; Takara, Dalian, China), 0.6 μl of each primer, 0.4 μl of rTaq polymerase (250 U, Takara), and 1 μl of DNA template. ..

Article Title: Molecular Phylogeny and Evolutionary History of the Genus Cyprinus (Teleostei: Cypriniformes)
Article Snippet: .. PCR amplifications were carried out in 50 μL reaction mixtures consisting of 5 μL 10 × PCR buffer (TaKaRa Biotechnology Co. Ltd., Dalian, China) 0.2 mM Fishes 2025, 10, 121 6 of 14 dNTPs, 0.2 μM of each primer, 1.5 U Taq DNA polymerase (TaKaRa Biotechnology Co. Ltd., Dalian, China), and approximately 50 ng genomic DNA, filled to 50 μL with sterilized water. .. Amplification was implemented as follows: denaturing at 94 ◦C for 5 min; 35 cycles of denaturation at 94 ◦C for 1 min; annealing at 52 ◦C for 1 min; and extension at 72 ◦C for 1 min. PCR products were electrophoresed in a 1.5% agarose gel, and successful amplifications were sequenced in an ABI Prism 3730 (Applied Biosystems Inc., ABI) automatic sequencer.

Review

rt pcr buffer (TaKaRa)

Structured Review

Images

Related Articles

Polymerase Chain Reaction:

Concentration Assay:

Amplification:

Sterility:

Random Hexamer:

Similar Products

Image Search Results